Modeling of high cell density fed batch cultivation

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture

Microbial lipid production by the oleaginous yeast Rhorosporidium toruloides Y4 was studied using glucose as carbon source, in order to realize igh-density cell culture. Batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l−1. Flask ed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g...

Batch and High Cell Density Fed-batch Culture Productions of an Organophosphorus Hydrolase

Organophosphorus hydrolases (OPH) are of great interest to the U. S. Army for their potential use in the non-toxic, non-corrosive decontamination against VX. OPH genes had been cloned and over expressed in Escherichia coli by other researchers. However, a common problem has been that of a low expression level. In this study, batch cultures in both complex (e.g., Lauria Broth) and minimal (succi...

High-cell-density cultivation of yeasts on disaccharides in oxygen-limited batch cultures.

Many facultatively fermentative yeast species exhibit a "Kluyver effect": even under oxygen-limited growth conditions, certain disaccharides that support aerobic, respiratory growth are not fermented, even though the component monosaccharides are good fermentation substrates. This article investigates the applicability of this phenomenon for high-cell-density cultivation of yeasts. In glucose-g...

Genetic Algorithms Based Parameter Identification of Yeast Fed-Batch Cultivation

Different kinds of genetic algorithms have been investigated for a parameter identification of a fermentation process. Altogether eight realizations of genetic algorithms have been presented four of simple genetic algorithms and four of multi-population ones. Each of them is characterized with a different sequence of implementation of main genetic operators, namely selection, crossover and muta...